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M94A2683.TXT
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1994-10-25
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Document 2683
DOCN M94A2683
TI A newly-developed immunofluorescence assay for simultaneous detection of
antibodies to human immunodeficiency virus type 1 and type 2.
DT 9412
AU Tanabe-Tochikura A; Singh MT; Tsuchie H; Zhang J; Mattunda SM; Paladin
FJ; Kurimura T; Dept. of Pathology, Osaka Univ., Suita, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):249 (abstract no. PB0424). Unique
Identifier : AIDSLINE ICA10/94369892
AB OBJECTIVE: Immunofluorescence assay (IFA) which is capable of
simultaneously detecting antibodies against closely related HIV-1 and
HIV-2 was established and evaluated for the ability to serologically
discriminate HIV-1 and HIV-2 infections. METHODS: IFA employing three
types of cells, HIV-1-infected (U937/LAI), HIV-2-infected
(Multinucleated giant cells which were formed by cocultivation of
MOLT-4#8 and MOLT-4/GH-1) and uninfected (HeLa) cells whose morphology
varies within one field of microscopy was performed. Serum specimens
from 95 HIV-1-infected, 26 HIV-2-infected and 3 HIV-1/HIV-2
dually-infected individuals and 106 seronegative blood donors were
tested using above mentioned cells. RESULTS: All the samples from
individuals infected with HIV were confirmed to contain antibodies to
HIV-1 and/or HIV-2. None of the sera from blood donors were positive.
Serum specimens from HIV-1-infected or HIV-2-infected individuals were
diagnosed as infections with HIV-1 (85/95) and HIV-2 (22/26),
respectively. Other 14 (10/95 and 4/26) were shown to be seropositive
for both HIV-1-infected and HIV-2-infected cells. DISCUSSION AND
CONCLUSION: Our results of newly-developed IFA was capable of
distinguishing most (88%) of HIV-1 and HIV-2 infections at one time.
This assay appears to be useful for screening as well as for confirming
of both HIV-1 and HIV-2 infections simultaneously.
DE AIDS Serodiagnosis/*METHODS Cell Line *Fluorescent Antibody Technique
Giant Cells/MICROBIOLOGY Hela Cells Human HIV Antibodies/*BLOOD HIV
Seropositivity/*DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY
HIV-2/*IMMUNOLOGY Predictive Value of Tests MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).